PHYLOGENETIC ANALYSIS OF UKRAINIAN ISOLATE OF RASPBERRY LEAF BLOTCH VIRUS
نویسندگان
چکیده
Raspberry leaf blotch virus (RLBV) is a new member of the Emaravirus genus (Fimoviridae, Bunyavirales). Representatives this family are characterized by segmented "-" RNA genome, helical nucleocapsid and enveloped spherical or pleomorphic virions 80-120nm in diameter. Transmission these viruses carried out eriophyid mites. The widely distributed Europe. In paper, phylogenetic relationships between Ukrainian RLBV isolate isolates from other countries GenBank database were investigated. Samples symptomatic raspberry plants selected for work. Total was isolated RT-PCR performed using primers to region protein (P3) gene. resulting amplicon with length about 500bp sequenced. Sequences analyzed BLAST MEGA7 programs. As result analysis, it shown that has high similarity some Finnish, British, Serbian Slovak (93-99% similarity). Nevertheless, dendrogram constructed did not distribute separate cluster. Interestingly, sequences Finland, Britain, Balkans segregated into different parts tree. This pattern can be explained low divergence population small number database.
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ژورنال
عنوان ژورنال: Agrofor
سال: 2021
ISSN: ['2490-3434', '2490-3442']
DOI: https://doi.org/10.7251/agreng2101019p